MEGA (Molecular Evolutionary Genetics Analysis) version X
Suliman Qadir Afridi
Virologist
Leipzig
Summary
Over 8 years of research experience in virology, bacteriology, molecular biology, and diagnostics across academia and industry. Skilled in assay development, filter testing for pathogen transmission, and vaccine development for emerging viral infections like COVID-19. Proficient in managing interdisciplinary projects with diverse collaborators."
Overview
11
11
years of professional experience
10
10
years of post-secondary education
8
8
Certifications
5
5
Languages
Work History
Scientist Vaccine Technology
Fraunhofer-Institut Für Zelltherapie Und Immunologie IZI
2 2020 - 2 2024
- Leading interdisciplinary projects and mentoring master's students.
- Conducting assay development and validation adhering to FDA and EU standards.
- Evaluating filter performance with nebulized virus particles, accounting for environmental dynamics.
- Contributing to upstream/downstream processing and vaccine development.
- Developing SOPs and defining lifecycle validation for analytical test methods.
- Ensuring compliance with FDA, EU, and ICH guidelines.
- Managing method transfers and authoring Analytical Method Validation protocols.
- Participating in troubleshooting and CAPA processes.
- Conducting immunoassays for protein detection and ensuring data protection compliance.
- Maintaining safety standards in genetic engineering and animal handling (FELASA B certified).
- Proficient in RT-PCR, protein expression, western blotting, ELISA, and cell culture assays.
- Familiarity with EU-GMP regulations and expertise in paper writing, patent searches, and project proposals."
Postdoctoral Researcher
Institute of Virology, Technische Universität München
10.2018 - 01.2020
- Developing SARS-CoV-2 vaccine and analyzing immune responses in COVID-19 patients.
- Validating serological assays for SARS-CoV-2.
- Identifying norovirus capsid protein epitopes responsible for immune escape.
- Producing norovirus VLPs and conducting crystallography in immunocompromised patients.
- Characterizing T-cells stimulated by norovirus VLPs.
- Conducting experiments under GLP.
PhD Researcher - Medical life science and technology
Institute of Virology, Technische Universität München
10.2014 - 10.2018
- To investigate evolutionary changes in norovirus of chronically infected patients, a quantitative ELISA was developed and used next generation sequencing and structural modeling with molecular dynamics simulation
- NGS (PacBio) R programing, Deseq2
- Production of recombinant norovirus capsid genes through molecular cloning to develop quantitative ELISA
- Differential analysis of norovirus VP1 to study changes in structure of sequential samples from immunocompromised patients.
Visiting Scientist
Li Ka Shing, Institute of Virology, University of Alberta
07.2018 - 08.2018
- Application of novel therapeutic approaches to control and eradicate HCV infection
- Live cell imaging using time lapse microscopy
- Cell culture of HEK293 line.
Microbiologist
Ideal Medical Laboratory and Research Centre Ltd
09.2012 - 01.2014
- Identification of different pathogens using real time PCR, conventional PCR, quantitative PCR and Nested PCR, blood chemistry
- Clinical chemistry, drug level determinations, endocrinology and HIV / hepatitis serology
- Blood serology
- Implementation of comprehensive legal and institute quality assurance systems.
Senior Medical Technologist (part time)
The Medical Laboratory Ltd
02.2010 - 03.2013
- DNA extraction and genotyping of HCV and other pathogens
- Hands-on experience of different analyzer i.e
- Architect, Modular, Elycsis analyzer
- Detection of HIV, HBV, HCV, HDV, H.pylori and STDs via ELISA.
Research Associate
Quality Operation Laboratory, UVAS
12.2010 - 06.2011
- To standardize analytical techniques (column chromatography) for detection of Salmonella and campylobacter spp
- In poultry meat and Egg
- Identification of different toxins in dairy products through chromatography
- Drug level determinations.
Research Assistant
National Institute of Biotechnology and genetic engineering (NIBJE)
06.2009 - 08.2009
- Preparation of media and culturing and purification of different novel fungal strains for biodegradation of coal.
Intern
Postgraduate Medical Institute, Hayatabad Medical Complex
09.2008 - 10.2008
- Identification of different pathogens through laboratory diagnosis
- Antimicrobial susceptibility tests and sterilization of different lab equipment's.
Education
PhD - Medical life science and technology -
Institute of Virology, Technische Universität München
10.2014 - 05.2019
M.Phil. Microbiology - undefined
University of Veterinary and Animal Sciences
08.2010 - 05.2012
B.Sc. (Hons) Microbiology - undefined
Kohat University of Science and Technology
Kohat, KPK 10.2005 - 05.2009
Skills
Personal Qualifications
PhD
Honors And Awards
- 07/27/2018, Best poster presentation, 11th International Conference on Virology and Microbiology, Vancouver, Canada
- 2014 - 2018, PhD Scholarship, German Academic Exchange Service (DAAD)
- 2008, English Access micro scholarship Program, U.S Mission Pakistan
Software
BLAST (Basic Local Alignment Search Tool)
Pymol v27 software
I-TASSER 50, PROSA-Web, GROMACS 455 COACH-D
Latex, EndNote and Mendeley for manuscript writing and reference management
GraphPad Prism for statistical data plotting
Adobe i lustrator, CorelDraw, BioRender and Milanote for data i lustrations
MS office especialy MS Excel- graphic tools, calculators, developing macro
Certification
Bioluminescent Technologies for studying Protein Biology and Cellular responses, Promega GmBH.
Photography, Traveling, Hiking, Reading
Photography: Capturing moments and exploring different perspectives through photography.
Traveling: Exploring new cultures, cuisines, and landscapes, broadening perspectives and experiences.
Hiking: Enjoying the great outdoors, exploring nature trails, and staying active.
Reading: Engaging in literature across various genres, expanding knowledge, and fostering creativity.
Timeline
Bioluminescent Technologies for studying Protein Biology and Cellular responses, Promega GmBH.
05-2023
Training for the person wearing breathing apparatus in filtering respiratory protection, Fraunhofer IZI, Leipzig, Germany.
04-2023
Scientists need more, Fraunhofer IZI, Leipzig, Germany.
02-2023
Data protection according to DSGVO, Fraunhofer IZI, Leipzig, Germany.
02-2023
Safety in Genetic Engineering, accordance to § 28 Abs. 5 in with Abs. 2 clause 1 Nr. 3 of the genetic engineering safety ordinance. Leipzig university, Germany
03-2022
Corruption prevention for a new employee, Fraunhofer IZI, Leipzig, Germany.
02-2022
FELASA B, Fraunhofer IZI, Leipzig, Germany.
02-2022
Introduction to Good Manufacturing Practice
02-2022
Postdoctoral Researcher
Institute of Virology, Technische Universität München
10.2018 - 01.2020
Visiting Scientist
Li Ka Shing, Institute of Virology, University of Alberta
07.2018 - 08.2018
PhD Researcher - Medical life science and technology
Institute of Virology, Technische Universität München
10.2014 - 10.2018
PhD - Medical life science and technology -
Institute of Virology, Technische Universität München
10.2014 - 05.2019
Microbiologist
Ideal Medical Laboratory and Research Centre Ltd
09.2012 - 01.2014
Research Associate
Quality Operation Laboratory, UVAS
12.2010 - 06.2011
M.Phil. Microbiology - undefined
University of Veterinary and Animal Sciences
08.2010 - 05.2012
Senior Medical Technologist (part time)
The Medical Laboratory Ltd
02.2010 - 03.2013
Research Assistant
National Institute of Biotechnology and genetic engineering (NIBJE)
06.2009 - 08.2009
Intern
Postgraduate Medical Institute, Hayatabad Medical Complex
09.2008 - 10.2008
B.Sc. (Hons) Microbiology - undefined
Kohat University of Science and Technology
10.2005 - 05.2009
Scientist Vaccine Technology
Fraunhofer-Institut Für Zelltherapie Und Immunologie IZI
2 2020 - 2 2024
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